Study on Plant Tissue Culture Techniques of Mulberry Tissue Culture

Mulberry is a perennial woody plant belonging to the deciduous tree species, mainly distributed in temperate and subtropical regions. China's fruit mulberry is mainly distributed in Jiangsu, Guangdong, Guangxi, Hubei, Shaanxi and other places. However, the scale is not large, generally not more than 33 hm2 mu per planting area. There are more than a dozen fruit mulberry varieties. Genetically speaking, there are diploid (2n), triploid (3n), and tetraploid (4n). Yield 2 250 kg/hm2, 7 500~11 250 kg/hm2, 30 000 kg/hm2. Mulberry seedlings have many methods of reproduction, which are broadly divided into sexual reproduction and asexual reproduction.
Grafting method, cutting method, first, slow, and second, poor quality, can not be detoxified, and susceptible to viral disease. In the past 20 years, with the continuous improvement and improvement of biotechnology, tissue culture has rapidly propagated and produced a large number of virus-free seedlings to solve the degradation of certain plants, increase fruit yield and quality, and apply them to production to speed up the cultivation of mulberry fine varieties. Open up new ways. Fruit mulberry not only has high yield of mulberry fruit, but also the quality and yield of mulberry leaf are equivalent to that of mulberry (common mulberry tree for silkworm rearing). Therefore, both the sericulture industry and the mulberry fruit can be harvested. Fresh mulberry can be directly supplied to the market. It can also be processed into mulberry juice, mulberry jam, mulberry fruit wine, mulberry wine, and mulberry kernel cake. It can also be used to purify food products such as food coloring and medical flavonoids. The Hong Kong magazine named Guosang as one of the third generation of popular fruits.
1 Materials and Methods 1. 1 Selection and treatment of materials Take top buds or lateral buds of robust shoots, cut off the leaves, scrub clean with sterile water, sterilize with sterile 0.1% solution of mercury for 10 min, sterile Rinse 3 to 4 times with water and place the treated tissue in the tissue culture chamber to start the culture.
1.2 Inoculation Under aseptic conditions, the apical buds or lateral buds are cut into 3 to 5 mm long sections, and the outer scales and lobule of the stem tips are stripped and inoculated onto the culture medium, and each bottle receives 5 to 7 segments.
1.3 Cultivation The apical buds or stem segments were inoculated in MS + 0.5 mg/L BA + 0.1 mg/L NAA + 0.1 mg/L GA medium supplemented with 3% sucrose and solidified with 0.5% agar. At about 25°C and under light, single shoots grew after 5 days, and after 4 weeks the shoots grew to 1 cm and clustered seedlings were produced.
Clustered seedlings were inoculated in MS + 2 mg/L BA + 0.5 mg/L GA medium, supplemented with 3% sucrose, and solidified on 0.5% agar.
When the cultured seedlings grow to a height of 3 to 5 cm, they can be cut into small sections from the internodes for subculture, and the lateral shoots sprout and grow rapidly. It can grow to 3~5cm in height in one month, and the subculture can make the lateral buds continuously proliferate, thus obtaining a large number of plantlets that can be propagated.
The shoots with the apical buds were excised and cultured in medium supplemented with 1/2 MS + 1 mg/L GA + 2% sucrose. After about 4 or 5 weeks, under natural light, roots develop into complete plants for transplanting.
3 Results and discussion This material is a dicotyledonous plant. During the cultivation process, we found that the incision in the stem section, especially the base incision, grew brownish similar to the callus and continued to inflate until it surrounded the bottom. This gradually yellows and shrinks from the top bud, causing culture to fail. Therefore, it is recommended that an appropriate amount of activated carbon and other absorbents be added to the medium to adsorb part of the browning material, and it is also possible to prevent the browning material from being transferred to a fresh medium or by removing the browning tissue. This has a certain effect.


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